Recommendations for the collection and delivery of urine according to the Nechiporenko method

For the medical diagnosis of illness of the urinary system, numerous research study techniques are utilized, amongst which is the Nechiporenko test. The analysis permits you to precisely recognize illness in severe and persistent types, which is why it is so typical in medical practice. Read more about the analysis of the results of a urine test according to Nechiporenko and how to gather it, read this short article.

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The story of

The author of this research study method is Alexander Zakharovich Nechiporenko, researcher, Soviet urologist-oncologist, military cosmetic surgeon, instructor. While studying the urine of clients, a researcher as soon as drew attention to its “unevenness” – every day the urine was various, its quantitative and qualitative structure altered.

Therefore, he made the following proposition: it is essential to take urine samples taking into consideration its everyday modifications – in the early morning, gathering just the middle part for research study (the initially and last couple of milliliters were expected to be drained pipes into the toilet).

Why did the Soviet urologist recommend this specific method? The truth is that the initially and last part of urine might include sediment from the bladder and flush from the urethra, and the physician had an interest in discovering concealed swellings of the urinary system.

The aspects discovered in the middle part of urine are counted according to an unique method, which is comparable to the system for counting blood aspects. The acquired indications assist to detect the urinary system, discover abnormalities in its work.


  • Preparing the client for urine collection is simple, and the method does not need a lot of labor from the medical personnel.
  • The research study method is easy, the outcomes are not inferior in dependability to other techniques, the analysis is performed fairly rapidly.
  • A percentage of biomaterial is required for research study.
  • Allows you to quickly display the characteristics of the pathological procedure, display the efficiency of treatment.
  • Helps to recognize swelling in slow types of illness.

How is the sample taken

The research study algorithm has actually not altered for several years. First, the gathered biological fluid is blended, then it is centrifuged, the upper layer is drawn off.

Leave 1 ml of the sample together with the sediment for analysis, mix and start to research study utilizing a counting chamber. The analysis takes numerous hours, so the outcomes are normally all set the next day.

As explained above, the Nechiporenko research study method is utilized as a diagnostic one. Usually it is recommended after a basic analysis (if any variances have actually been recognized in the indications) in order to acquire a more in-depth photo for the medical diagnosis from the gathered information.

Also, this analysis is done to display the efficiency of the treatment. The information is analyzed as follows:

  • The standard of leukocytes is up to 2000 per 1 ml, if there are more of them, then an inflammatory procedure happens. Increased rates are observed in prostatitis, pyelonephritis, urolithiasis, cystitis.
  • The standard of erythrocytes is up to 1000 in 1 ml. If the indications are greater, then there is every factor to presume a major swelling with bleeding.
  • According to the developed standard, 1 ml of urine can include up to 20 hyaline cylinders. If more of them are discovered, then for sure swelling is actively establishing in the kidney hips.

It is very important to comprehend that just a medical professional can properly comprehend the analysis. Trying to separately discover by analysis whether there is cystitis or not is not worth it – a thorough evaluation is required to make a medical diagnosis. In addition, the results can be incorrect (if the client did not understand how to gather the urine properly or it was not transferred properly).

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What illness assists to detect

  • Pyelonephritis. There are a lot of red cell and leukocytes, there are germs and pus.
  • Cystitis. An increased concentration of leukocytes and erythrocytes, cells of polymorphic cystic epithelium, germs is figured out in the sediment.
  • Acute glomerulonephritis. Hyaline casts exist, waxy and granular casts can be observed. There are more leukocytes than erythrocytes.
  • Chronic glomerulonephritis. This illness is defined by the predominance of the number of erythrocytes over leukocytes.

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Many more illness can include to this list. If any variances are exposed, extra tests are normally recommended.

  • Chronic cholecystitis medical standards.
  • Preparations after cholecystectomy.


  • You do not require to consume any tablets (or other medications) prior to gathering urine as they might impact the results. If you cannot cancel their reception, you require to notify your physician about it. There are cases that cystitis due to medication cannot be figured out by tests, although there are signs.
  • Before gathering a urine test according to Nechiporenko, it is essential to rinse with warm water, otherwise germs that have actually collected on the external genital organs might wind up in a container with urine. This can lead to incorrect readings.
  • Do not utilize any gels or foams for cleansing, particularly with anti-bacterial action. If even a tiny part of such funds enters the container, this can lead to the death of some of the microbes. Outcome — the research study outcomes are misshaped. If, nonetheless, common moderate soap is utilized, then you require to clean it off extremely well with water, and then clean it dry.
  • The day prior to taking urine according to Nechiporenko, it is not suggested to consume foods which contain a lot of natural dyes (carrots, beets). You need to likewise avoid spicy and spicy foods, consuming alcohol.

If the preparation for the collection of the sample was broken, notify your physician about it. To acquire trusted outcomes, he can hold off the delivery of the analysis to another day.

How much urine requirements to be gathered

Of course, the volume of urine launched might vary from individual to individual, depending upon the drinking program, the frequency of clearing the bladder, the attributes of the body, illness (for example, cystitis forces an individual to urinate in little parts).

Sometimes the quantity of urine gathered is so little that it is difficult to perform a research study – the lab cannot evaluate its structure, and for that reason it is essential to gather urine once again. A sensible concern occurs – just how much urine is required for analysis.

The needed volume depends upon the particular analysis. Usually, 50-100 ml of urine is taken for research study, this quantity can be examined by a lab assistant. There are research studies for which urine for analysis is gathered all the time, then the volume of urine contributed can reach 2 liters.

You can discover just how much urine the Nechiporenko test needs from your physician. As a guideline, 20–35 ml suffices (the middle part is gathered). Considering that urine collection according to Nechiporenko requirements to be carried out in the early morning, even children need to not have troubles – the correct amount of fluid will gather in the bladder over night.

How much urine is required for analysis in babies: 5-10 ml, about the exact same quantity of urine the infant produces at a time. Of course, it will not be simple for medical professionals to deal with such a volume, however research study is possible.

How to take a sample from grownups

The guidelines for gathering urine according to Nechiporenko are easy: a typical part is offered, make certain to in the early morning. It is suggested to gather it in unique containers, sterilized containers for delivery can be purchased the drug store.

Before taking urine tests according to Nechiporenko, they are cleaned with warm water (females need to clean so that the motions are from front to back, this will avoid germs from the intestinal tracts from getting in the urethra and vaginal area).

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After the end of the health treatments, the genital areas are cleaned dry with a tidy towel and the urine is gathered for analysis in a ready meal. The container needs to not touch the body. When the client has actually gathered the needed part (a minimum of 20-35 ml), the container is securely closed with a cover and right away sent out to the lab.

For the result to be trusted, the period in between taking a sample and its analysis need to disappear than 2 hours, so it is suggested to provide the gathered part to the lab within an hour. If the biological fluid is kept longer, its pH will increase and foreign germs might pollute it. Urine can be transferred in the fridge at above-zero temperature levels, it cannot be frozen.

How to properly contribute urine for Nechiporenko analysis if there is no sterilized container: you can take a little glass container and an ideal cover, wash and sanitize them completely, and then dry them.

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Is it possible to take a sample throughout menstruation

On menstrual days, urine is normally not contributed, as blood can enter it. It is much better to take a urine test according to Nechiporenko 2-3 days after the end of menstruation, alerting your physician about this. But there are times when research study cannot be held off.

How to take a urine test according to Nechiporenko with menstrual bleeding: prior to taking an analysis, females are encouraged to place a gynecological tampon into the vaginal area, and then urinate.

How to take a sample from kids

The algorithm is the like for grownups: initially you require to completely clean yourself with warm water without soap.

In order not to blockage the sample with unneeded germs, when cleaning, kids require to relocation the foreskin and expose the head of the penis, ladies – to push the labia apart and wash so that the water streams from front to back (otherwise germs from the anal area can enter the urethra, vaginal area).

If the kid is accustomed to going to the toilet on his own, he needs to be advised, discussing all the guidelines for cleaning and how to appropriately gather the analysis.

How to properly pass urine tests according to Nechiporenko for kids who do not yet go to the toilet by themselves: as a guideline, it is seldom recommended to pass urine according to Nechiporenko to children under 3 years of ages, given that it is tough to gather urine from them properly. More typically they gather the overall part in unique bags-urine collectors, which are taken a look at.

The urine ejected of diapers, diapers is not appropriate. Also, for urine analysis according to Nechiporenko, it is not encouraged to gather urine from the pot – even if it is cleaned extremely well, microbes might stay that will misshape the results.

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The Nechiporenko analysis is an important research study method for identifying swelling, it is utilized in the medical diagnosis of lots of persistent and severe illness of the urinary system and is utilized to track the efficiency of treatment. The algorithm for its execution is easy, and in terms of the dependability of the results, it is not inferior to other techniques of studying urine.

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Svetlana Borszavich

General practitioner, cardiologist, with active work in therapy, gastroenterology, cardiology, rheumatology, immunology with allergology.
Fluent in general clinical methods for the diagnosis and treatment of heart disease, as well as electrocardiography, echocardiography, monitoring of cholera on an ECG and daily monitoring of blood pressure.
The treatment complex developed by the author significantly helps with cerebrovascular injuries and metabolic disorders in the brain and vascular diseases: hypertension and complications caused by diabetes.
The author is a member of the European Society of Therapists, a regular participant in scientific conferences and congresses in the field of cardiology and general medicine. She has repeatedly participated in a research program at a private university in Japan in the field of reconstructive medicine.